The running principle of peptide synthesis

Solid phase reaction principle of peptide synthesis, amino acids in explosion-proof glass in a closed reactor in a known order (sequence, usually from c-n-terminal end to n-carboxy-terminal end) added, reaction, synthesis, operations, a final peptide carrier. Solid-phase synthesis, significantly reduce the difficulty of each step of product purity. In order to prevent the occurrence of side effects, and participate in the reaction of the amino acid side chains are protected. C-Terminal is free, and must be activated before the reaction. Solid-phase synthesis method, there are two, namely Fmoc and tBoc. Due to the Fmoc tBoc has many advantages, now mostly synthesized using Fmoc method, but for some short peptides, tBoc because of their high yield advantage was still being used by many companies.
synthesis by the following loops:
1) to protect: Fmoc-protected post and unit must be in an alkaline solution (piperidine) removal of amino protecting groups.
2) activation and cross-linking: activated for the next amino acids the carboxyl group is an activating agent. Activated monomer reacts with free amino cross-linking to form peptide bonds. This step uses a lot of hyper-driven reaction concentration reagent. Cycle: synthesis of these steps repeatedly until completed.
3) Elution and deprotection: peptide eluting from the column down, its protected groups in a deprotection reagent (TFA) Elution and deprotection.